منابع مشابه
Application of Gel Chromatography*
INTRODUCTION The term Gel Chromatography refers to the process of separation of solutes caused by the passage of a solution through a column filled with a porous gel. The dissolved particles may enter the pores fully or partially or they may remain completely excluded. This results in a sorting out of the dissolved particles according to their size, so that the largest particles migrate with th...
متن کاملGel-Filtration Chromatography.
Gel-filtration chromatography is a versatile method that permits the effective separation of biological molecules in high yield. This article describes the basis of the method, the selection of suitable operating conditions, and contrasts typical matrix types. Applications of the technique are described, with references to the scientific literature.
متن کاملFractionation of human urine by gel chromatography.
High-resolution anion-exchange chromatography of human urine can resolve more than 150 constituents that absorb ultraviolet light. Prefractionation of urine on Sephadex G.10 helped us identify the constituents and simplified the anion-exchange chromatogram. The material associated with the 13 peaks obtained by gel chromatography was pooled and concentrated into six fractions, which were subsequ...
متن کاملHigh Performance Gel Permeation Chromatography of Polymers
The determination of the molecular weight distribution by gel permeation chromatography requires the establishment of a molecular weight calibration and efficient columns in order to minimise chromatogram broadening. The separation process is described in terms of steric exclu— sion, retardation and partial exclusion mechanisms in order to show how the universal calibration method may be influe...
متن کاملMeasurement of DNA-protein equilibria using gel chromatography: application to the HinfI restriction endonuclease.
A method is described for measuring equilibrium constants of DNA-protein interactions using gel chromatography. This technique has been used to study the sequence-specific interaction of the HinfI restriction endonuclease with DNA. HinfI has a monomeric molecular weight of 31000 and exists as a dimer in its active form. The protein binds to supercoiled DNA molecules containing its recognition s...
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ژورنال
عنوان ژورنال: Pure and Applied Chemistry
سال: 1969
ISSN: 1365-3075,0033-4545
DOI: 10.1351/pac196920030309